|
KMID : 0614020040190010029
|
|
Journal of Pharmaceutical Sciences (C.N.U.)
2004 Volume.19 No. 1 p.29 ~ p.33
|
|
|
Lectin Activities of 32 Korean Wild Basidiomycetes Including Thelephora terrestris
|
|
Shin Ka-Young
Lee Ji-Seon
Kim Yong-Joo
Kim Bit-Na
Chung Kyeong-Soo
|
|
|
Abstract
|
|
|
|
As a preliminary step to develop noble immunomodulatory lectins from Korean basidiomycetes, 32 species of wild mushrooms were collected and their lectin activities were screened using hemagglutination assay. As a result, cold-water extract of 21 species including Thelephora terrestris showed agglutination activities From T. terrestrisa, partially purified lectin fractions, cTTL_(50) and cTTL_(100), were prepared by extraction with cold phosphate-buffered saline (pH 7.2), precipitation with ammonium sulfate and then dialysis Of these. cTTL_(100) was found to be a strong immunomodulating lectin in that it aggluinated BALB/c erythrocytes and sarcoma 180 tumor cells at the concentration of ≥1.95 ㎍/㎖ and ≥39 ㎍/㎖, respectively, and stimulated the peritoneal macrophages to spread at 200 ㎍/㎖.
|
|
|
KEYWORD
|
|
|
Basidiomycetes, lectin, hemagglutination, Thelephora terrestris, macrophage spreading, immunomodulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|